Subcutaneous Vaccine Administration Procedure

Click here for Subcutaneous Vaccine Administration Procedure training video.

  1. Always have enough people (two at minimum) and use proper animal restraint to administer the vaccine safely. Adverse vaccine reactions can be minimized if the vaccine is warmed in the hand to close to body temperature.
  2. Gently grasp and lift a fold of skin to tent the skin over the proper location.
  3. Insert the needle into the fold of skin. Ensure that the needle is into the subcutaneous space but not poking through the other side of the fold.
  4. Aspirate by drawing back on the syringe plunger. You should encounter some resistance. If you draw back and aspirate anything other than a small bubble of air (trapped in the needle hub):
    • Remove the needle from the skin.
    • Check needle for proper seal on syringe. Replace the needle if defective.
    • Reinsert needle into the skin.
    • Never continue the vaccination process if blood is drawn back into the hub.
  5. Depress the plunger, slowly injecting the vaccine. If a large amount of resistance is encountered, reposition in the subcutaneous space and attempt to reinject.
  6. When the vaccine is completely injected, remove the needle from the skin.
  7. Dispose of needle in the sharps container.
  8. Dispose of the syringe and vaccine vials in the biohazard bin.
  9. Check the injection site immediately afterward for any blood or spilled vaccine.
  10. Gently massage the vaccine area to disperse the vaccine.

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